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Cytometry and flow cytometry of 4′,6‐diamidino‐2‐phenylindole (DAPI)‐stained suspensions of nuclei released from fresh and fixed tissues of plants
Author(s) -
Sgorbati Sergio,
Levi Marisa,
Sparvoli Elio,
Trezzi Federica,
Lucchini Giovanni
Publication year - 1986
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1986.tb03384.x
Subject(s) - dapi , flow cytometry , pisum , biology , sativum , microbiology and biotechnology , staining , fluorescence , botany , genetics , physics , quantum mechanics
Cytometry and flow cytometry were used to study characteristics of fluorescence of the DNA‐DAPI complex in nuclei released from different fresh and formaldehyde‐fixed pea ( Pisum sativum L. cv. Lincoln) tissues. The two methods of isolation are compared and discussed as well as their possible use for quantitative analysis of DNA in plant tissues. With fixed tissues it is possible to obtain a number of nuclei sufficient for the flow cytometric analysis, even using small amounts of plant tissue.