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Endogenous levels of abscisic acid, indole‐3‐acetic acid, zeatin and zeatin‐riboside during the course of adventitious root formation in cuttings of Craigella and Craigella lateral suppressor tomatoes
Author(s) -
Maldiney R.,
Pelèse F.,
Pilate G.,
Sotta B.,
Sossountzov L.,
Miginiac E.
Publication year - 1986
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1986.tb03376.x
Subject(s) - zeatin , abscisic acid , hypocotyl , cutting , auxin , primordium , biology , cytokinin , gibberellin , horticulture , botany , indole 3 acetic acid , chemistry , biochemistry , gene
Indole acetic acid (IAA). abscisic acid (ABA), and zeatin plus zeatin riboside (Z + ZR) were determined daily in cuttings of Lycopersicon esculentum Mill. cv. Craigella (C) and the Craigella Lateral Suppressor (CLS) mutant during the first 5 days of the root‐forming process. A solid‐phase enzyme immunoassay using specific anti‐hormone antibodies was used following a one‐step HPLC purification procedure. The hormone measurements were made in cuttings divided into 4 parts. The main variations occurred in the terminal bud and in the basal part of the hypocotyl of the two tomato varieties, i.e. significant IAA and ABA increases during the first 2 days followed by a more or less fast return to the initial values at day 4 or 5. This is probably due to the ablation of the root system. Z + ZR levels dramatically decreased in the basal part of the Craigella hypocotyl 1 day after cutting. Contrary to CLS, C hypocotyls recovered the initial high levels when roots regenerated (day 5). This is probably linked to the greater ability of roots to produce cytokinins in C plants than in CLS ones. The first step of root formation (reactivation of the pericyclic cells and formation of root primordia) corresponded to a high IAA/Z + ZR ratio in the root‐forming tissue, whereas the second step (elongation of young roots) was characterized by a low ratio with low levels of hormones.