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Isolation and partial characterization of latex lectins from three species of the genus Euphorbia (Euphorbiaceae)
Author(s) -
NsimbaLubaki Makuta,
Allen Anthony K.,
Peumans Willy J.
Publication year - 1986
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1986.tb02442.x
Subject(s) - euphorbia , euphorbiaceae , fucose , biochemistry , asparagine , mannose , biology , carbohydrate , lectin , glycoprotein , molecular mass , amino acid , chemistry , botany , enzyme
Three N‐acetylgalactosamine‐specific lectins were isolated from the latices of Euphorbia calcina L., Euphorbia dalberi L. and Euphorbia sp. (an undetermined species) by affinity chromatography on fetuin‐agarose. They are all glycoproteins [about 12.5% (w/w) carbohydrate] of M, around 140 000 and appear to be tetrameric molecules composed of different subunits. All three lectins have similar amino acid (with high contents of asparagine/aspartic acid, giycine and leucine) and carbohydrate (with glucosamine, mannose.) fucose and xylose) compositions. In addition. they are closely related serologically.