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Sucrose release from soybean leaf slices
Author(s) -
Anderson James Michael
Publication year - 1986
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1986.tb02427.x
Subject(s) - sucrose , chemistry , glycine , carbohydrate , biochemistry , horticulture , botany , food science , biology , amino acid
The release of photosynthate from leaf slices of soybean [ Glycine max (L.) Merr. cv. Ransom II], to a bathing medium was studied to ascertain how p ‐chloromercuribenzenesulfonic acid (PCMBS) can both stimulate and inhibit sucrose release. Soybean leaf slices released photosynthate to a bathing medium at a rate that was approximately linear with time. The photosynthate released was about 20% ionic and 80% non‐ionic, and sucrose represented about 75% of the total. Removal of Ca 2+ from the medium increased the rate of release of all fractions, but amino acid release showed the largest increase. Sucrose was released at a rate estimated to be about 20% of the normal transport rate in intact leaves. The rate of sucrose uptake from 5 m M sucrose into soybean leaf slices was optimum at pH 6.3, and the rate of sucrose release was lowest at the same pH. However, sucrose uptake was found to be insignificant during release experiments. Sucrose release, but not amino acid release, was inhibited 75% by 1 m M PCMBS. The data support two components of sucrose release in leaves. The first is insensitive to the addition of PCMBS. This component probably represents leakage from phloem tissue. The second component is inhibited by PCMBS and probably represents release from the mesophyll. By comparing sucrose release from leaf slices of 12 different species of plants, 2 groups were found. In the first group, sucrose release was inhibited between 60 and 80% by PCMBS, and in the second group between 0% and 40%. The difference in the two groups can be explained by a relative difference in the size of the two components of sucrose release for each species.