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Isomers of zeatin and zeatin riboside in clubroot tissue: evidence for trans‐zeatin biosynthesis by Plasmodiophora brassicae
Author(s) -
Müller Peter,
Hilgenberg Willy
Publication year - 1986
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1986.tb02415.x
Subject(s) - zeatin , cytokinin , brassica , clubroot , biology , high performance liquid chromatography , biochemistry , chemistry , chromatography , botany , auxin , gene
Cytokinin‐like substances in both healthy and infected ( Plasmodiophora brassicae ) Wor. strain S) roots of Brassica campestris L. ssp. pekinensis cv. Granat have been tentatively identified and quantified by HPLC. The isomers of the cytokinins could be seperated on a reversed phase column using a gradient elution with increasing amounts of methanol. Secondary plasmodia were isolated mechanically from Plasmodiophora brassicae infected roots. The time course of adenine uptake and its conversion to cytokinins were investigated. Evidence is presented for the incorporation of [U‐ 14 C]‐adenine into trans ‐zeatin by secondary plasmodia.