Premium
A method for determining the apoplastic water volume of conifer needles
Author(s) -
Beeson R. C.,
Montano J. M.,
Proebsting W. M.
Publication year - 1986
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1986.tb01244.x
Subject(s) - apoplast , picea abies , volume (thermodynamics) , pinus <genus> , chemistry , botany , beech , horticulture , biology , physics , thermodynamics , cell wall
A method for direct estimation of percentage apoplastic water volume (% APO) in conifer needles is described. The method presented here, and designated the pressure‐needle (P‐N) method, measures the relative water content of the needles to develop a curve similar to the pressure‐volume (P‐V) curve. P‐V and P‐N curves were developed for Picea pungens Engelm. cv. Hoopsi, Pinus sylvestris L., Abies gradis (Dougl.) L., and Pseudotsuga menziesii (Mirb) Franco. The % APO estimated by the two procedures varied as much as 2‐fold, while other parameters were similar. The P‐V method generated consistently higher and more variable % APO than the P‐N method, due to the inclusion of the apoplastic water of the stem in the P‐V method. For conifers, the P‐N method offers a more accurate and precise method for determining % APO.