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Microculture of single protoplasts of Brassica napus
Author(s) -
Spangenberg G.,
Koop H.U.,
Lichter R.,
Schweiger H. G.
Publication year - 1986
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1986.tb01223.x
Subject(s) - brassica , protoplast , microculture , biology , division (mathematics) , botany , osmotic concentration , cell division , horticulture , cell culture , biochemistry , genetics , cell , arithmetic , mathematics
Protoplasts of Brassica napus L. were cultured individually in a microdroplet system using a synthetic medium with survival rates of more than 70% and division frequencies of up to 65%. Microcallus formation occurred at frequencies of up to 50%. Factors affecting the survival and division of individually cultured protoplasts, such as composition and volume of culture medium, pH, buffering system, osmolarity and genotype, were analyzed.