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sse of photosynthetic parameters for the diagnosis of copper deficiency in Pinus radiata seedlings
Author(s) -
Gorgé J. López,
Lastra O.,
Chueca A.,
Lachica M.
Publication year - 1985
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1985.tb08682.x
Subject(s) - photosynthesis , chlorosis , botany , copper , thylakoid , horticulture , photosystem ii , seedling , chlorophyll , chemistry , electron transport chain , pinus radiata , chloroplast , biology , biochemistry , organic chemistry , gene
Six‐month‐old water cultures of Pinus radiataI D. Don seedlings showed optimal growth, and the highest CO 2 assimilation and photosystem I‐dependent ascorbate/dichlorophenolindophenol → NADP + electron flow, at 3.0 uM Cu 2+ (excess) in the hydroponic media. In the nine‐month‐old water cultures, when the early Cu deprivation has been overcome, the optimum for plant growth and CO 2 fixation shifts to 0.3 u M Cu 2+ (normal); at that time, the 3.0 uM Cu 2+ water cultures showed toxic symptoms of foliar chlorosis. Under Cu 2+ deficient levels (0.03 uM) a clear decrease in the photosystem I‐linked electron transport and CO 2 assimilation rates, as well as in the whole plant development, could be observed. Both six‐ and nine‐month‐old water cultures showed a close relationship between the Cu 2+ concentration of the media and the foliar Cu content. However, leaf chlorophyll and the Cu content of thylakoid lamellae showed such a correlation only in the Cu 2+ deficient and Cu 2+ normal water cultures. The conclusion from these results is that the electron transport rate ascorbate/dicblorophenolindophenol → NADP + , and the Cu content of the photosynthetic membranes, can be used to diagnose a Cu deficiency in Pinus radiata plants.