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Vitamin D 3 affects growth and Ca 2+ uptake by Phaseolus vulgaris roots cultured in vitro
Author(s) -
Vega Manuel A.,
Santamaria Eduardo C.,
Morales Alfonsina,
Boland Ricardo L.
Publication year - 1985
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1985.tb08667.x
Subject(s) - phaseolus , ionophore , cycloheximide , elongation , calcium , vitamin , epicotyl , biology , biochemistry , chemistry , protein biosynthesis , seedling , botany , materials science , organic chemistry , ultimate tensile strength , metallurgy
Vitamin D 3 at low concentration (10 −9 M) inhibited the growth of Phaseolus vulgaris L. (cv. Contrancha) roots in vitro as measured by elongation (14 h) and [ 3 H]‐leucine incorporation into protein (2 h), and increased their labelling with 45 Ca 2+ (2 h). Cycloheximide and puromycin (50 u.M) blocked vitamin D 3 stimulation of root 45 Ca 2+ labelling, indicating that it is mediated by de novo protein synthesis. The calcium ionophore X‐537A (10 −5 JW) induced similar changes both in root elongation and 45 Ca 2+ uptake (14 h). This may indicate that the inhibitory effects of the sterol on root growth are mediated by changes in Ca 2+ fluxes. However, this interpretation should be further strengthened by additional studies as the ionophore may have acted on root growth, affecting physiological processes other than Ca 2+ transport.