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Carbon dioxide and ethylene evolution in the culture atmosphere of Magnolia cultured in vitro
Author(s) -
Proft Maurice P.,
Maene Ludo J.,
Debergh Pierre C.
Publication year - 1985
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1985.tb08660.x
Subject(s) - carbon dioxide , atmosphere (unit) , ethylene , explant culture , in vitro , tissue culture , botany , gas phase , chemistry , biology , chromatography , horticulture , biochemistry , organic chemistry , physics , thermodynamics , catalysis
Subcultured explants of Magnolia soulangeana Soul, were incubated in tissue culture containers fitted with different types of closures. Type of closure affected the CO 2 concentration, with levels as high as 14% CO 2 being detected. The ethylene concentration increased gradually with time, to as much as 2–3 ppm after 9 weeks. There was a large variation in the composition of the atmosphere within the containers of any one type. The way by which a container was closed influenced exchange of the inner gas phase with the surrounding atmosphere and was important in determining the development of the cultured tissues.