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Regulation of nitrate and nitrite reduction in barley leaves
Author(s) -
Soares M. Inês M.,
Lips S. Herman,
Cresswell Cristopher F.
Publication year - 1985
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1985.tb08528.x
Subject(s) - nitrite , nitrate , chemistry , hordeum vulgare , dcmu , anaerobic exercise , biochemistry , botany , photosynthesis , biology , poaceae , photosystem ii , physiology , organic chemistry
Reduction of nitrate and accumulation of nitrite were studied in barley (Hordeum vulgare L. cv. Gars Clipper ex Napier) leaf sections in the dark and in the light, under aerobic (air and mixtures of O 2 and N 2 ) or anaerobic (N 2 ) conditions. Oxygen prevented nitrite accumulation but had no effect on accumulated or infiltrated nitrite. Most of the nitrite accumulated under dark‐anaerobic conditions was in the “cytoplasmic” (the cell section between the plasma lemma and the tonoplast) fraction of the tissue. Reduction of nitrate was stimulated by 2, 4‐dinitrophenol in tissue under dark‐air and by 3‐(3′, 4′‐dichlorophenyl)‐l, l‐dimethyl urea (DCMU) and carbonyl cyanide m ‐chlorophenylhydrazone (CCCP) in tissue under all environmental conditions studied. Nitrite accumulated in the light in DCMU‐treated tissue under N 2 or under aerobic conditions in the presence of CCCP. On its own, CCCP did not promote accumulation of nitrite in leaf sections under light‐air. A model for the reduction of nitrate and nitrite is proposed.