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DNA changes involving repeated sequences in senescing soybean ( Glycine max ) cotyledon nuclei
Author(s) -
Chang DauYin,
Miksche Jerome P.,
Dhillon Sukhraj S.
Publication year - 1985
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1985.tb03361.x
Subject(s) - cotyledon , biology , dna , repeated sequence , microbiology and biotechnology , nuclear dna , dna sequencing , satellite dna , genetics , biochemistry , gene , genome , mitochondrial dna , botany
Age‐related DNA changes in soybean ( Glycine max L. cv. Ransom) cotyledon nuclei were investigated by Feulgen cytophotometry and cloned DNA probes. The amount of nuclear DNA in 17‐day‐old senescing cotyledons was 23% lower than that of 5‐day‐old young cotyledons. In order to understand the nature of senescence‐related DNA loss, fragments of repetitive DNA from young cotyledons were cloned into Escherichia coli HB101 cells by DNA recombination. The cloned DNA probed changes in specific repeated nucleotide sequences in senescing cotyledons. The colony hybridization between cloned DNA and [ 32 P]‐labeled total DNA from 5‐ and 17‐day cotyledons indicated loss of specific repeated sequences. The selected sequences of repeated DNA further showed a loss in their copy numbers. The study suggested that some repetitive DNA sequences were degraded selectively in the genome of senescing cotyledons.