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Initiation and development of wound tissue and roots on hypocotyl cuttings of Pinus sylvestris in vitro
Author(s) -
Grönroos Roland,
Arnold Sara
Publication year - 1985
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1985.tb03359.x
Subject(s) - cutting , tracheid , hypocotyl , botany , charcoal , biology , activated charcoal , pinus <genus> , chemistry , xylem , adsorption , organic chemistry
The rooting of hypocotyl cuttings from 20‐day‐old seedlings of Pinus sylvestris L. cultured in vitro is discussed. About 40% of the cuttings cultured on medium lacking activated charcoal produced roots during the first two months. When activated charcoal was added to the medium, either root formation (75% formed roots) or wound tissue growth (95% formed large wound tissues) was stimulated in different experiments. These large wound tissues did not develop any roots. The anatomical changes in the basal part of the cuttings were similar during the first two weeks in all the cuttings studied. A vascular cylinder composed of short tracheids with many pores developed. Thereafter the differentiation process became varied. The amount of wound tissue produced and the time for rooting differed among the cuttings. Tracheid nests which were in contact with the vascular system in the hypocotyl via short tracheids were observed after three weeks. Subsequently, roots developed from the tracheid nests. The longer root formation was delayed, the larger the wound tissue became. Short tracheids were found close to the wound tissue surface. Their ability to adsorb nutrients and water is discussed.