Premium
Chlorate‐resistant rose cells: influx, efflux and reduction of [ 36 Cl]‐chlorate
Author(s) -
Murphy Terence M.,
Wrona Anne F.,
Wycoff Keith
Publication year - 1985
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1985.tb03350.x
Subject(s) - chlorate , chemistry , nitrate reductase , nitrate , nuclear chemistry , salt (chemistry) , inorganic chemistry , botany , biology , organic chemistry
Clones of Rosa damascena Mill. cv. Gloire de Guillan, selected for growth in solid medium containing 56 m M NaClO 3 , were studied to determine the reason for their resistance to this toxic salt. The cells grew on medium containing nitrate as the only nitrogen source, and they synthesized nitrate reductase (EC 1.6.6.2) in the presence of nitrate. The cells were resistant in the presence of nitrate. However, their resistance was greatly increased by the presence of glutamate in the medium. The cells took up [ 36 Cl]‐ClO 3 ‐ and reduced it to ClO 2 − , but the fraction of ClO 3 − that they reduced under our experimental conditions was less than that reduced by wild type. The slower production of ClO 2 − apparently accounted for the resistance of the cells to ClO 3 − . We suggest several possible reasons for the low rate of reduction of ClO 3 − .