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Is lipoxygenase involved in the formation of ethylene from ACC?
Author(s) -
Kacperska Alina,
KubackaZgbalska Maria
Publication year - 1985
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1985.tb03349.x
Subject(s) - lipoxygenase , ethylene , chemistry , linoleic acid , incubation , stimulation , linolenic acid , biochemistry , substrate (aquarium) , polyunsaturated fatty acid , enzyme , fatty acid , food science , biology , catalysis , ecology , neuroscience
Freezing or desiccation of winter rape leaves ( Brassica napus L. var. oleifera (cv. Górczanski) stimulated both lipoxygenase (EC 1.13.11.12) activity and ethylene formation during the post‐stress period. The effect depended on the degree of membrane injury. In tissues showing injury less than 50% (as checked with the electrical conductivity method) both activities increased according to the degree of stress‐induced damage. In leaves injured to a higher degree both activities decreased. Light and low temperature (5°C) inhibited the development of both lipoxygenase activity and ethylene formation in leaf disks stored for 20 h. Ethylene formation was also observed in a model system where soybean lipoxygenase was added to a mixture containing 1‐aminocyclopropane‐1‐carboxylic acid and linoleic or linolenic acid as substrate for lipoperoxide formation. Changes in pH and temperature conditions of the incubation mixture caused similar differences in the lipoxygenase activity and ethylene formation. We propose that the stimulation of lipoxygenase‐catalysed oxidation of polyunsaturated fatty acids (increasing free radical formation) leads to an increased ethylene production from ACC.