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Intracellular adenylate pools and protein degradation during zoosporangial differentiation in Allomyces arbuscula
Author(s) -
Ojha Mukti,
Turian Gilbert
Publication year - 1985
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1985.tb02824.x
Subject(s) - energy charge , adenylate kinase , intracellular , biology , zoospore , germination , protein degradation , mycelium , biochemistry , ammonium , metabolism , botany , spore , enzyme , chemistry , organic chemistry
In Allomyces arbuscula Butl., strain Bali, the ratio of protein to dry weight remained constant in exponentially growing but decreased in differentiating cultures. The adenylate pools (ATP, ADP, AMP) and energy charge which integrates them, increased during zoospore germination and stabilized around 0.9 during differentiation. The level of ATP increased early during the induction of zoosporangia for up to 1 h and then declined. The ADP and AMP remained low for most of the time except for a transient increase in ADP (first 30 min induction). The energy charge was low in spores. The rate of turnover of proteins during growth and differentiation was more or less similar for up to 1.5 h after transfer. Subsequently very little turnover of proteins occurred in the growing plants. In differentiating plants, the rate of degradation was maintained and by the end of the 4 h experimental period 30% of the vegetative proteins were degraded. The intracellular ammonium showed a peak between 30 to 60 min of induction and was higher in the differentiating mycelia than in actively growing plants, while the glutamate pool remained around 1 μmol (mg protein) −1 in both types of plants. The physiological role of these protein degradation products is discussed.