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Efficiency of respiration and energy requirements of N assimilation in roots of Pisum sativum
Author(s) -
Visser Ries
Publication year - 1985
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1985.tb02384.x
Subject(s) - pisum , respiration , carbohydrate , sativum , biology , rhizobium leguminosarum , botany , leghemoglobin , symbiosis , photosynthesis , biochemistry , root nodule , horticulture , rhizobiaceae , bacteria , genetics
The function of alternative path respiration in roots was investigated in pea plants ( Pisum sativum L. cv. Rondo). Plants were grown in symbiosis with Rhizobium leguminosarum (strain PF2), completely dependent on N 2 fixation, or non‐nodulated, receiving nitrate or ammonium at the same rate as N 2 was fixed in symbiosis. Under these conditions, relative growth rates of plants grown with N 2 , NO ‐ 3 or NH + 4 were the same. This facilitated interpretation of the effect of the N source on the efficiency of root respiration, as determined by the relative activity of the non‐phosphorylating alternative path. The ‘wasteful’ oxidation of carbohydrate via this pathway was defined as the glucose equivalent of the difference between the amounts of ATP (mol O 2 ) ‐1 produced in cytochrome and alternative path respiration. ‘Wasteful’ carbohydrate oxidation maximally amounted to 4% (N 2 ), 15% (NO ‐ 3 ) and 25% (NH + 4 ) of the daily carbohydrate oxidation in the roots. It is concluded that the ‘wasteful’ oxidation of carbohydrate via the alternative path is of minor importance for the adaptation of root respiratory metabolism to different energy requirements of N assimilation. The total carbohydrate import by roots fixing N 2 was ca 60 and 30% higher than the import by roots assimilating NO ‐ 3 or NH + 4 , respectively. Two factors are shown to account for these differences: the high carbohydrate cost of N 2 fixation, and the small contribution (30%) of the roots to NO ‐ 3 reduction by the plant. The high carbohydrate requirements of roots fixing N 2 were met by higher rates of photosynthesis as compared with plants utilizing NO ‐ 3 or NH + 4 .