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Effects of NaCl on the properties of phosphoenolpyruvate carboxylase from Suaeda monoica and Chloris gayana
Author(s) -
ShomerIlan Adiva,
MoualemBeno Delila,
Waisel Yoav
Publication year - 1985
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1985.tb02362.x
Subject(s) - chloris gayana , phosphoenolpyruvate carboxylase , phosphoenolpyruvate carboxykinase , substrate (aquarium) , halophyte , enzyme , botany , biochemistry , biology , chemistry , salinity , ecology , dry matter
The effects of NaCl on the kinetic properties of desalted phosphoenolpyruvate carboxylase (PEP carboxylase, EC 4.1.1.31) from two halophytes, Suaeda monoica Forssk. ex. J.F. Gmel and Chloris gayana Kunth. were investigated. The tolerance of PEP carboxylase to NaCl in the reaction medium depends on the enzyme pre‐conditioning as well as on the concentration of its substrate PEP in the assay medium. Addition of PEP to the extraction and the storage medium, stabilizes the enzyme. Such a pre‐treated enzyme is inhibited by NaCl in the presence of low concentrations of PEP in the assay medium but is activated by NaCl in the presence of PEP at concentrations above 1.0 m M . NaCl modifies the n H value, K’ and V max , and seems to act as an allosteric effector.