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Changes in intracellular UDP‐sugar levels during the cell cycle in a synchronous culture of Catharanthus roseus
Author(s) -
Amino Shinichi,
Takeuchi Yuichi,
Komamine Atsushi
Publication year - 1985
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1985.tb02335.x
Subject(s) - catharanthus roseus , arabinose , sugar , xylose , galactose , biochemistry , intracellular , chemistry , chromatography , fermentation
UDP‐sugar contents were measured using high performance liquid chromatography and gas chromatography during the cell cycle in a synchronous culture of Catharanthus roseus (L.) G. Don. UDP‐glucose, UDP‐galactose, UDP‐glucuronic acid, UDP‐xylose and UDP‐arabinose could be determined, and 75–90% of the UDP‐sugars were UDP‐glucose. The contents of UDP‐glucose and UDP‐galactose increased in the late G2‐M and the late S‐M phases, respectively, whereas UDP‐glucoronic acid and UDP‐arabinose increased in amount in the G1 phase. These changes in the levels of UDP‐sugars during the cell cycle generally correlated well with the changes in cell wall constituents and in the activities of the enzyme involved in synthesis and interconversion of UDP‐sugars reported by S. Amino et al. (Physiol. Plant. 1985. 64: 111–117).