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Effects of saponin extracts from Solanum elaeagnifotium fruits on ion uptake by clover seedlings
Author(s) -
Agüero Marta S.,
Boland Ricardo L.
Publication year - 1985
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1985.tb01909.x
Subject(s) - saponin , chemistry , in vitro , chromatography , atpase , centrifugation , botany , biochemistry , enzyme , biology , medicine , alternative medicine , pathology
Solanum elaeagnifolium Cav. fruits contain high concentrations of steroidal saponins. Treatment of 3‐day‐old clover seedlings with aqueous fruit extracts modified Ca 2+ uptake without significantly altering K + and H 2 PO 4 − uptake. The extracts increased Ca 2+ uptake in the concentration range of 0.2 to 20 m M Ca 2+ . Uptake curves could be represented by two phases. In the lower phase (0.2‐1.0 m M Ca 2+ ), this change could be related to an increase in V max . Pretreatment of seedlings with saponin extracts significantly reduced ATP‐dependent Ca 2+ uptake and Ca 2+ ‐dependent ATPase activity in a fraction isolated from root homogenates by centrifugation at 1500 g for 15 min. Saponins purified from S. eleagnifolium extracts by thin‐layer chromatography modified in vitro the Ca 2+ ‐ATPase activity of this fraction, indicating that the steroid may act directly on Ca 2+ transport across membranes.