Premium
Anatomical observations on somatic embryogenesis from scutellar tissues of immature zygotic embryos of Triticum aestivum
Author(s) -
Magnusson Ingrid,
Bornman Chris H.
Publication year - 1985
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1985.tb01893.x
Subject(s) - scutellum , zygote , embryo , somatic embryogenesis , biology , botany , somatic cell , cell division , microbiology and biotechnology , anatomy , embryogenesis , genetics , cell , gene
Somatic embryos were observed as early as six days after subculturing immature embryos of Triticum aestivum L. (cvs Froid‐Centurk and Helge) in vitro on 2,4‐dichloro‐phenoxyacetate‐containing nutrient media. Embryo formation followed three pathways, each involving one of the scutellum's three basic tissue systems: dermal, ground and vascular. (1) Single epithelial layer cells divided tangentially to give pseudothallus‐like structures which, through radial and oblique divisions, assumed polar, proembryoid symmetry. (2) In actively dividing ground tissues, localized asymmetrical division in some cells resulted in proembryoids. When contiguous with each other, the proembryoids could be identified as a proembryonic mass. (3) Oblique divisions in some cells of the scutellum's procambium resulted in daughter cells of unequal size, from the smaller of which the embryoid's root would eventually form.