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Uptake of benzyladenine in explants of Picea abies and Pinus sylvestris
Author(s) -
Vogelmann Thomas C.,
Bornman Chris H.,
Nissen Per
Publication year - 1984
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1984.tb06365.x
Subject(s) - picea abies , explant culture , pinus <genus> , micropropagation , botany , horticulture , chemistry , karst , in vitro , biology , biochemistry , paleontology
Primary explants of Picea abies (L.) Karst. and Pinus sylvestris L. were incubated for various periods in solutions containing different concentrations of [ 14 C]‐benzyladenine. Time course analysis showed that uptake was linear for the initial 60 min; after this time linear uptake continued but at a much reduced rate. The amount of benzyladenine taken up by the explants saturated at a concentration about one third of that of the medium, Concentration dependence experiments showed that BA uptake was directly proportional to the external concentration. These results and those from experiments in which uptake was examined at different temperatures are consistent with a passive mode of BA uptake. The results are discussed with respect to in vitro micropropagation.