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Inhibition of gravitropism in oat coleoptiles by the calcium chelator, ethyleneglycol‐ bis ‐(β‐aminoethyl ether)‐N′‐tetraacetic acid
Author(s) -
Daye Sun,
Biro Ronald L.,
Roux Stanley J.
Publication year - 1984
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1984.tb06354.x
Subject(s) - egta , gravitropism , coleoptile , calcium , avena , biophysics , chemistry , biochemistry , botany , biology , organic chemistry , arabidopsis , mutant , gene
A treatment period as brief as 8 h in 10 −3 M EGTA completely blocks gravitropism in 70–80% of the treated coleoptiles of oats ( Avena sativa , L. cv. Garry) without inhibiting growth. Only about 10% of the plants perfused in water failed to exhibit gravitropism. Subsequent perfusion of EGTA‐treated plants with calcium completely restores gravitropism; post‐perfusion with water does not. After perfusion in water for 10 h, gravistimulated oat coleoptile segments show the same asmmetry of 45 Ca distribution as reported earlier for non‐perfused coleoptiles and sunflower hypocotyls. The degree of this asymmetry is reduced in those coleoptiles partially completely inhibited by perfusion in EGTA and is essentially absent in those coleoptiles completely inhibited by EGTA. The fact that calcium reverses the inhibitory effects of EGTA on gravitropism indicates that the inhibition was probably due to a reduction in the availability of free calcium required for one or more of the transduction steps of gravitropism.