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The development of Pisum sativum explant systems for studies concerning source‐sink activities
Author(s) -
Achhireddy N. R.,
Kirkwood R. C.,
Berg G.
Publication year - 1984
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1984.tb06112.x
Subject(s) - pisum , sucrose , sativum , shoot , gibberellic acid , anthesis , chromosomal translocation , stipule , biology , botany , horticulture , chemistry , biochemistry , cultivar , germination , gene
Using excised fruit/shoot systems of pea ( Pisum sativum L.), the effect of the 10‐day‐old (post anthesis) fruit (sink) on the translocation of 14 C‐sucrose applied to the stipule (source) was investigated. We also examined the influence of various growth regulators on the source‐sink relationships using this system. Indole‐3‐yl‐acetic acid (100 or 200 ppm), gibberellic acid (25‐100 ppm) increased 14 C‐sucrose translocation into the growing fruit by about 13% and 22–29%, respectively. 2‐chloroethyl phosphonic acid, 6‐benzyladenine and 2‐methyl‐4‐chlorophenoxyacetic acid also increased 14 C‐sucrose translocation into the fruit by about 11–26%, 11–23% and 24–37%, respectively. Elevated fruit 14 C‐sucrose levels found in other treatments include, methyl‐2‐chloro‐9‐hydroxyfluorene‐9‐carboxylate (300 or 1000 ppm), 2,3,5‐triiodobenzoic acid (100 and 300 ppm) and butanedioic acid mono‐(2,2‐dimethylhydrazide) (30‐300 ppm) by about 34%, 39–52% and 7–13%, respectively.