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Control of root and shoot‐bud formation from potato tuber tissue cultured in vitro
Author(s) -
Kikuta Yoshio,
Okazawa Yozo
Publication year - 1984
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1984.tb06092.x
Subject(s) - shoot , kinetin , zeatin , biology , sucrose , mannitol , botany , solanum tuberosum , murashige and skoog medium , explant culture , axillary bud , in vitro , horticulture , cytokinin , auxin , biochemistry , gene
Comparative studies on the control of root and shoot‐bud formation and plant regeneration have been undertaken in discs (1 × 6 mm in diameter) excised from tubers of potato ( Solanum tuberosum L. cv. Irish Cobbler) cultured in vitro. The results clarified that the optimal culture conditions for shoot‐bud formation were quite different from those for root formation and, in conclusion, that (1) shoot‐buds were produced when cultured in modified White's medium containing 0.25 M mannitol with 2.3 μ M zeatin and 0.57 μ M indole‐3‐acetic acid (IAA) at 20°C under relatively high light irradiation, while (2) roots were readily formed when cultured in modified White's medium containing 29 m M sucrose with 4.7 μ M kinetin plus 1.7 μ M IAA at 30°C in darkness.