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A study of phytochrome‐membrane association using an enzymelinked immunosorbent assay and Western blotting
Author(s) -
Jordan Brian R.,
Partis Michael D.,
Thomas Brian
Publication year - 1984
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1984.tb06085.x
Subject(s) - phytochrome , blot , avena , in vivo , membrane , biology , recombinant dna , chemistry , microbiology and biotechnology , biochemistry , botany , red light , gene
Enzyme‐linked immunosorbent assay (ELISA) and Western blotting have been used to study the association between phytochrome and membranes in dark‐grown tissue of Avena sativa L. cv. Saladin. Enhanced phytochrome pelletability can be demonstrated by ELISA after red irradiation in vivo and extraction in buffer containing Mg 2+ , but not with red irradiation of Mg 2+ alone. This enhanced association may, however, be restricted to specific membranes. The results obtained with ELISA are confirmed by Western blotting techniques. Membrane‐associated and soluble phytochrome give different immunological responses and it is suggested that immunologically distinct populations of phytochrome may exist in dark‐grown tissue.