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An enzyme‐linked immunosorbent assay for phytochrome
Author(s) -
Thomas Brian,
Crook Norman E.,
Penn Susan E.
Publication year - 1984
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1984.tb06084.x
Subject(s) - phytochrome , avena , enzyme , antiserum , biochemistry , biology , enzyme assay , chemistry , antibody , botany , red light , immunology
A double‐antibody sandwich, enzyme‐linked immunosorbent assay has been developed for phytochrome in Avena sativa L. cv. Saladin. An immunoglobulin fraction of rabbit antiserum raised to 118 kdalton phytochrome was used with alkaline phosphatase as the enzyme label. The assay detected as little as 0.2 ng phytochrome in extracts of dark‐grown plant material. No evidence for specific or non‐specific measurement of proteins other than phytochrome was found. The assay detected phytochrome in extracts of Avena grown in the light. Dilution curves for light‐grown phytochrome extracts had a reduced slope and saturated at a lower level of enzyme activity than those for dark extracts. These differences were not caused by an inhibitor in extracts from light‐grown plants. Phytochromes from dark‐ and light‐grown plants may be immunologically different.