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Chromophore structure in phytochrome intermediates and bleached forms of phytochrome
Author(s) -
Thümmler F.,
Rüdiger W.
Publication year - 1984
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1984.tb06079.x
Subject(s) - chromophore , phytochrome , etiolation , ethylene glycol , avena , chemistry , photochemistry , glycerol , aqueous solution , urea , biochemistry , red light , organic chemistry , botany , biology , enzyme
Phytochrome (120 kdalton or 60 kdalton) was isolated from etiolated seedlings of Avena sativa L. cv. Pirol (Baywa München). Irradiation with red light of the P r form at −23°C in aqueous medium or at −40°C in 66% glycerol leads to the intermediate meta‐Rb. Acidification of the glycerol solution at −40°C leads to the absorption of the 15(E) phytochrome chromophore (= P fr chromophore). Subsequent irradiation transforms this into the 15(Z) chromophore (= P r chromophore). The presence of the 15(E) chromophore was demonstrated by the same methods also in phytochrome bleached either as P fr in the dark by 4 M urea, methanol, acetone, ethylene glycol, 8‐anilinonaphthalene‐1‐sulfonate, or as P r by irradiation with red light in the presence of the same agents. Phytochrome bleached by sodium dodecylsulfate or by dehydration was also investigated. It was concluded that bleached phytochrome contains the P fr chromophore without specific interaction with the protein.