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Cytofluorometric measurements of nuclear DNA in adventitious buds and shoots of Picea abies regenerated in vitro
Author(s) -
Hakman I.,
Arnold S.,
Bengtsson A.
Publication year - 1984
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1984.tb06070.x
Subject(s) - ethidium bromide , shoot , biology , botany , protoplast , nuclear dna , picea abies , dna , in vitro , staining , biochemistry , genetics , gene , mitochondrial dna
Nuclear DNA content of adventitious buds and shoots of Picea abies (L.) Karst. developed in vitro was compared to that of buds collected from field‐grown trees. Protoplasts were isolated from the different tissues and after fixation and staining with ethidium bromide the DNA content of their interphase nuclei was determined cytofluorometrically. The DNA pattern of the different tissues was within the same range and had the same distribution with one main peak.