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Identification of the photosynthetic CO 2 fixation inhibitors in isolated pine chloroplasts as resin acids
Author(s) -
Martin Björn,
Malmberg Gunilla,
Gref Rolf,
Öquist Gunnar
Publication year - 1984
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1984.tb05932.x
Subject(s) - chloroplast , photosynthesis , chemistry , abietic acid , resin acid , carbon fixation , biochemistry , chromatography , organic chemistry , gene , rosin
Thus far all attempts to isolate CO, fixing chloroplasts from pine have failed. In this paper it is proposed that resin acids present in pine needles partition into membranes during chloroplast isolation and interfere with specific reactions of the Calvin cycle. CO, fixation by isolated spinach chloroplasts was strongly inhibited by the introduction of a suspension of chloroplasts isolated from Pinus sylvestris L. A partially purified organic extract obtained from chloroplasts of this pine species also strongly inhibited CO, fixation by the spinach chloroplasts. The major inhibitory compounds from the organic extract were identified as a mixture of resin acids by gas‐liquid chromatography and mass spectrometry. Two resin acids, abietic acid and dehydroabietic acid, were tested for inhibitory activity. Both resin acids were potent inhibitors of photosynthetic CO 2 fixation, with dehydroabietic acid being about three times more potent than abietic acid.