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Allantoinase and asparaginase activities in maturing fruits of nodulated and non ‐nodulated soybeans
Author(s) -
Gomes M. A. F.,
Sodek L.
Publication year - 1984
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1984.tb05931.x
Subject(s) - point of delivery , biology , asparagine , cotyledon , glycine , asparagine synthetase , botany , horticulture , enzyme , biochemistry , amino acid
Immature fruits of soybean ( Glycine max L. Merr. cv. Santa Rosa) were found to contain high ureide/amino acid ratios for plants dependent on atmospheric nitrogen (nodulated), but low ratios for plants cultivated on NO − 3 (non‐nodulated). The pod tissue was responsible for almost all this difference, which reflects the N metabolism of these plants (nodulated:urcide‐based; NO − 3 dependent: asparagine based). The capacity of fruit tissues to utilize ureides and asparagine via allantoinase (EC 3.5.2.5) and asparaginase (EC 3.5.1.1) was investigated during fruit development. Both enzymes were present in crude desalted extracts of all parts of the fruit analysed (pod, cotyledon and seed coat). Asparaginase was detected in pod tissue only at early stages and with very low activities, whereas high activities of allantoinase (up to 20 [imol pod −1 h −1 ) were present after this organ reached full expansion. The cotyledons contained most of the allantoinase and asparaginase activities of the seed, the highest activities being recorded during the period of rapid protein accumulation. There was little difference in the activity patterns for nodulated and NO − 3 ‐grown plants, despite the large difference in nitrogen nutrition of the fruits.