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Interaction of respiratory and photosynthetic electron transport, and evidence for membrane‐bound pyridine‐nucleotide dehydrogenases in Anabaena variabilis
Author(s) -
Stürzl Erwin,
Scherer Siegfried,
Böger Peter
Publication year - 1984
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1984.tb04915.x
Subject(s) - anabaena variabilis , dcmu , photosystem i , photochemistry , electron transport chain , photosynthesis , anabaena , photosystem ii , chemistry , nadph dehydrogenase , photosystem , electron acceptor , cyanide , biochemistry , biology , cyanobacteria , enzyme , bacteria , inorganic chemistry , genetics , nitric oxide synthase
Membranes isolated from vegetative cells of Anabaena variabilis (ATCC 29413) oxidine NADPH and NADH in a cyanide‐sensitive reaction, NADPH being the better donor. In addition, both act as reductants for photosystem I in the light and in the presence of DCMU. An NADH‐regenerating system has been introduced experimentally, functioning as donor system for photosystem I of Anabaena membranes. This NADH dehydrogenation is strictly light‐dependent, sensitive to DBMIB, establishes a transhydrogenase system with NADP + was hydrogen acceptor and competes with oxygen. Kinetic analysis of oxygen uptake with reference to nucleotide concentration exhibited different K m ‐ and V max ‐values for NADPH and NADH in the light and in the dark.