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Protease activities in non‐embryogenic and embryogenic carrot cell strains during callus growth and embryo formation
Author(s) -
Carlberg Irene,
Söderhäll Kenneth,
Glimelius Kristina,
Eriksson Tage
Publication year - 1984
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1984.tb04603.x
Subject(s) - protease , daucus carota , proteases , embryo , biology , biochemistry , callus , enzyme , botany , microbiology and biotechnology
Embryogenic and non‐embryogenic cell strains of Daucus carota L. were examined for their protease activity using a wide range of chromogenic synthetic peptides as substrates. High arginine‐specific activity was present in all strains, but no protease activity “specific” for embryogenic or non‐embryogenic strains could be detected with the substrates tested. The specific protease activity was 5–10 times higher in the non‐embryogenic as compared to the embryogenic strain for most tested substrates, and this difference was not due to release of proteases in the latter. All strains showed a decrease in protease activity when cultured in media without 2,4‐dichlorophenoxyacetic acid, but the embryos had high protease activity in comparison with the nondifferentiated cell aggregates. In the latter aggregates, hydrolyzing activity towards three of the substrates (H‐D‐Phe‐Pip‐Arg‐ p ‐nitroanilide, Suc‐Ala‐Pro‐Phe‐ p ‐nitro‐anilide and Bz‐Phe‐Val‐Arg‐ p ‐nitroanilide) was absent, whereas the embryos were able to hydrolyze them.