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Transcriptional activity of chromatin in developing and germinating triticale embryos
Author(s) -
Weidner Stanislaw,
Wielgat Bernard
Publication year - 1984
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1984.tb04554.x
Subject(s) - germination , anthesis , triticale , embryo , chromatin , biology , rna , ripeness , embryogenesis , caryopsis , botany , horticulture , microbiology and biotechnology , dna , ripening , biochemistry , cultivar , gene
Rythmical changes in the transcriptional activity of embryo chromatin during development and maturation of grain of triticale (cv. MT‐3) have been observed. The template availability and RNA polymerase (EC 2.7.7.6) 1 and II activities were high at the beginning of embryo development (20 to 30 days) and lowest at days 35 to 40 after anthesis, after which they increased to the end of maturation (60 days). Grains from three important periods of development (20, 40 and 60 days after anthesis; that is milk, wax and full ripeness, respectively) were used for germination, and chromatin was isolated from embryos and seedlings. A dramatic increase in the activity of both RNA polymerases and template availability was observed after 48 h of germination. The increase in template availability depended also on the ripeness stage of the grain used for germination.