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Protein and carbohydrate accumulation in normal and high‐lysine barley in spike culture
Author(s) -
Mather Diane E.,
Giese Henriette
Publication year - 1984
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1984.tb04253.x
Subject(s) - hordein , endosperm , sucrose , starch , hordeum vulgare , carbohydrate , chemistry , botany , dry matter , food science , biochemistry , poaceae , biology
Spikes of barley ( Hordeum vulgare L.) cultivar Bomi and high‐lysine mutants Riso 1508 and Riso 56 were cultured on liquid media at varying N and sucrose levels. Bomi accumulated N in response to increasing N levels in the medium and a higher level was reached than in spikes of intact plants. The distribution of N in salt‐soluble, hordein, and non‐protein N fractions appeared to be normal. Endosperm dry weight and starch were lower than in intact plants and declined at higher N levels. A linear relationship was observed between starch content and the concentration of sucrose in the endosperm water. Uptake of culture medium by the spikes was affected by both N and sucrose concentration. The mutants had lower dry weights and starch contents, and higher sucrose contents than Bomi. At high N levels, the mutants accumulated less hordein, and more non‐protein N than Bomi.