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Effects of carbon dioxide in anther cultures
Author(s) -
Johansson Lars,
Eriksson Tage
Publication year - 1984
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1984.tb04244.x
Subject(s) - anemone , biology , incubation , botany , clematis , sea anemone , microspore , stamen , embryo , traditional medicine , pollen , microbiology and biotechnology , biochemistry , medicine , herb , medicinal herbs
In anther cultures of Anemone canadensis L., Anemone dichotoma L., Anemone hupehensis Lemoine, Clematis viticella L. and Papaver setigerum DC. a positive relationship between incubation in 2% CO 2 and the production of microspore‐derived embryos was observed. In anther cultures of Nicotiana tabacum L., Anemone hupehensis and Clematis viticella a combination of cold treatment (7°C) and incubation in 2% CO 2 increased embryo production. In Anemone canadensis cold treatment increased the number of proembryos, whereas incubation in 2% CO 2 had no effect. In Anemone hupehensis 5% CO 2 increased embryo production by more than 2%. In Anemone dichotoma and Papaver setigerum 2% CO 2 was the more efficient level. CO 2 had no significant effect on pH in the culture medium in anther cultures of Anemone canadensis.