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Spectrophotometric quantitation of protochlorophyll(ide): Specific absorption and molar extinction coefficients reconsidered
Author(s) -
Kahn Albert
Publication year - 1983
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1983.tb06578.x
Subject(s) - molar absorptivity , protochlorophyllide , absorbance , absorption (acoustics) , extinction (optical mineralogy) , cucumis , hordeum vulgare , acetone , chemistry , analytical chemistry (journal) , chromatography , botany , biology , biochemistry , poaceae , mineralogy , optics , chloroplast , physics , gene
To allay doubt about how to calculate molar extinction eoeffieients from the specific absorption coefficients of protoehlorophyll(ide), dark‐grown leaf segments and saponin protochlorophyll(ide) holochrome subunits frotn barley ( Hordeum vulgare L.) and cotyledons of cucumber ( Cucumis sativus L.) were extracted with acetone without or following prior, brief illumination. Absorbance data and eoeffieients of chlorophyll a were used to derive extinetion eoeffieients of protoehlorophyll(ide); 626 nm (range: 30 to 35 1 mmol ‐1 cm ‐1 ) without recourse to published coefficients c protochlorophyll(ide). These results combined with evaluation of how the origin; coefficients were obtained, argue for using the molecular weight of protochlorophy rather than protochlorophyllide to calculate molar extinction eoeffieients from th specific absorption coefficients.