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Synchrony induced by double phosphate starvation in a suspension culture of Catharanthus roseus
Author(s) -
Amino Shinichi,
Fujimura Tatsuhito,
Komamine Atsushi
Publication year - 1983
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1983.tb04220.x
Subject(s) - catharanthus roseus , phosphate , cell division , dna synthesis , biology , cell cycle , mitosis , cell culture , cell growth , mitotic index , thymidine , suspension culture , botany , biochemistry , dna , cell , microbiology and biotechnology , genetics
A synchronous cell division system was established using the double phosphate starvation method, based on the observation that one of the limiting factors in the growth of a suspension culture of Catharanthus roseus (L.) G. Don cells in the medium of Murashige and Skoog was phosphate. In the system, an increase in cell number took place in a short period of only 4 h, while the cell number remained almost constant during other periods of the cell cycle. The synchrony of the culture was confirmed by changes in mitotic index, which increased sharply prior to the increase in cell number. The S phase was determined by measuring incorporation of [ 3 H]‐thymidine into the DNA fraction during the cell cycle and synchrony of DNA synthesis was verified likewise. Synchronization by phosphate starvation is discussed in relation to the function of phosphate as a nutrient. The synchronous system thus established will be useful in biochemical studies of the cell cycle in higher plants.