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The role of ethylene in the induction of amylase activity in wheat aleurone tissue
Author(s) -
Varty K.,
Arreguín L. B.,
Lopez T. P. J.,
Sanchez F. C. J.,
Garcia H. E.,
Gomez L. M. A.
Publication year - 1983
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1983.tb04187.x
Subject(s) - gibberellic acid , aleurone , ethylene , amylase , biochemistry , gibberellin , hordeum vulgare , chemistry , biology , enzyme , botany , germination , poaceae , catalysis
When wheat aleurone layers ( Triticum aestivum L. var. Potam S‐70) incubated in medium containing gibberellic acid were exposed to ethylene, the synthesis and release of amylase were enhanced relative to layers incubated in the presence of mercuric perchlorate. Exogenous ethylene stimulated gibberellic acid‐induced amylase synthesis by approximately 2.2‐fold. The ethylene‐mediated stimulation of amylase formation was dependent upon the tissue being exposed to the gas during the lag phase of gibberellic acid action. Ethylene appeared to promote only quantitative changes in amylase synthesis and release, since the isoelectric focusing patterns of amylase is enzymes were not significantly altered by ethylene. Ethylene had no effect on the incorporation of [methyl‐ 14 C]choline into aleurone phospholipids, but stimulated the accumulation of [U‐ 14 C]adenine into poly(A) RNA of gibberellic acid‐treated tissue by about 80%.