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Effect of cycloheximide on IAA‐induced proton excretion and IAA‐induced growth in abraded Avena coleoptiles
Author(s) -
Pope David G.
Publication year - 1983
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1983.tb04180.x
Subject(s) - coleoptile , avena , cycloheximide , excretion , iodoacetic acid , botany , chemistry , biology , biochemistry , enzyme , protein biosynthesis
IAA‐induced proton excretion in peeled or abraded oat ( Avena saliva L. cv. Victory) coleoptiles is closely associated with IAA‐induced growth. It was attempted to separate these two processes by using cycloheximide to inhibit them differentially. Growth of abraded coleoptile segments was measured by a shadow graphic method, and their IAA‐induced acidification of the external solution was monitored with a pH meter. IAA stimulated proton excretion in abraded Avena coleoptile segments after a 13 min lag. IAA‐induced proton excretion was inhibited within 5 min by cycloheximide at concentrations of 1.8 × 10 −6 , 3.6 × 10 or 3.6 × 10 −5 M. Cycloheximide at these concentrations, added within 4 min of IAA, prevented IAA‐induced acidification of the medium for at least 60 min. However, it did not prevent IAA‐induced growth during this time. It is concluded that some of the initial IAA‐induced growth seen in Avena coleoptiles is independent of detectable IAA‐induced proton excretion.