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Cherry fruit development: Indoleacetic acid oxidase isoenzymes in the seed
Author(s) -
Valpuesta Victoriano,
Bukovac Martin J.
Publication year - 1983
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1983.tb04171.x
Subject(s) - isozyme , oxidase test , chemistry , biochemistry , kinetics , polyacrylamide gel electrophoresis , biology , horticulture , enzyme , quantum mechanics , physics
Two anionic indoleacetic acid oxidase isoenzymes were separated by polyacrylamide gel electrophoresis from an acetate buffer (0.2 M, pH 4.0) extract of sour cherry ( Prunus cerasus L. cv. Montmorency) seed. One isoenzyme migrated to Rf 0.25 (I1) and the other to Rf 0.78 (I 2 ). Isoenzyme I, exhibited hyperbolic kinetics and was found during all three stages of fruit development with the highest levels during early stage II. The isoenzyme I 2 showed sigmoidal kinetics and was found only during stages II and III of fruit growth with highest levels during stage III. The activities of both isoenzymes were markedly enhanced by addition of Mn2+ and 2,4‐dichloro‐phenol to the reaction mixture. Isoenzyme I, showed higher affinity for indoleacetic acid than isoenzyme I 2 . The significance of these isoenzymes in cherry fruit growth is discussed.