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Effect of plant growth hormones and polyamines on ornithine decarboxylase activity during the germination of barley seeds
Author(s) -
Kyriakidis Dimitrios A.
Publication year - 1983
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1983.tb02775.x
Subject(s) - putrescine , ornithine decarboxylase , spermidine , cadaverine , polyamine , hordeum vulgare , spermine , biochemistry , abscisic acid , gibberellic acid , ornithine decarboxylase antizyme , biology , germination , chemistry , botany , enzyme , poaceae , gene
Gibberellic acid (GA 3 ) and β‐indolylacetic acid (IAA), two of the well known growth hormones, induce four fold the activity of ornithine decarboxylase (ODC) during the germination of barley seeds ( Hordeum vulgare L. var. Beca). The optimal concentration for induction of ODC was 10 –5 M for GA 3 and 10 –3 M for IAA. When 10 –3 M of a polyamine, putrescine or spermidine, is added to the growth medium, ODC activity is significantly inhibited. This inhibition is due to the induction of a protein inhibitor of ODC (antizyme), whose apparent molecular weight is 16 000 ± 2 000 daltons. Addition of GA 3 to cultures which have been grown for 50 or 98 h in the presence of polyamines, abolishes the observed inhibition of ODC activity, while in the reverse experiment, addition of polyamines at 50 or 98 h does not affect the ODC activity induced by GA 3 . Cadaverine, a physiological plant diamine, enhances ODC activity; whereas 1,8‐diaminooctane (the alkyl analogue of spermidine) does not have any effect.