Premium
Sugar uptake in soybean ( Glycine max ) cells in suspension culture
Author(s) -
KlerkKiebert Y. M.,
Kneppeis T. J. A.,
Matthijs H. C. P.,
Verieur J. D.
Publication year - 1983
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1983.tb00902.x
Subject(s) - glycine , biochemistry , sugar , chemistry , metabolism , respiration , dinitrophenol , glucose uptake , biology , amino acid , botany , insulin , endocrinology
In chlorophylkras soybean ( Glycine max L.) cell suspensioo cultures glucose uptake has been studied using the analogue 3‐O‐methyIglucose. Uptake could be distinguished into: a) a high affinity phase with K m = 0.06 m M and b) a low affinity phase with K m 2.0 m M . The uptake of glucose was accompanied by H + ‐cotransport with a stoichiometry of 0.3 H + per molecule 3‐O‐methylglucose. Experiments in which sugar uptake was measured in the presence of various inhibitors of respiration and photosynthesis demonstrated that the glucose uptake system was dependent on energy metabolism and the ATP‐content of the cells. Efflux experiments in the presence of the uncoupler dinitrophenol confirmed this energy dependency. Glucose uptake did not decrease before the ATP‐content of the cells had decreased considerably.