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The fate of carbon during the assimilation of carbamoyl phosphate in white spruce seedlings as revealed by [ 14 C]‐carbamoyl phosphate, [ 14 C]‐cyanate, and [ 14 C] ‐bicarbonate labeling patterns
Author(s) -
Durzan D. J.
Publication year - 1983
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1983.tb00764.x
Subject(s) - cyanate , bicarbonate , chemistry , carbamoyl phosphate synthetase , phosphate , urea , biochemistry , arginine , amino acid , assimilation (phonology) , carbamyl phosphate , dihydroxyacetone phosphate , metabolism , organic chemistry , enzyme , linguistics , philosophy
The fate of carbamoyl phosphate in white spruce seedlings revolves around the production of spontaneous degradation products, cyanate, bicarbonate, and carba‐mate. When [ 14 C]‐carbamoyl phosphate and [ 14 C]‐cyanate are assimilated, urea is a common early metabolic intermediate that appears in the alcohol soluble N. By contrast, urea is not detected among the products of [ 14 C]‐bicarbonate. Carbamoyl phosphate and glutamic acid are implicated as having pivotal roles in the production of amides, arginine, and biotin. Within 2‐h exposures to radioactive substrates considerably more carbon from bicarbonate was diverted into amino acids Incorporated into proteins than with carbon‐nitrogen substrates. Specific activities of bound amino acid residues support the view that proteins formed from these [ 14 C]‐substrates have different rates of metabolic turnover.