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Effects of NaCl salinity in vivo and in vitro on ribonuclease activity of Vigna unguiculata cotyledons during germination
Author(s) -
Filho Eneas Gomes,
Prisco José Tarquínio,
Paiva Campos Francisco de Assis,
Filho Joaquim Eneas
Publication year - 1983
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1983.tb00755.x
Subject(s) - vigna , cotyledon , salinity , germination , rnase p , sephadex , seedling , ribonuclease , enzyme assay , in vivo , enzyme , biology , biochemistry , botany , chemistry , rna , ecology , microbiology and biotechnology , gene
Pitiuba bean [ Vigna unguiculata (L.) Walp.] seeds were sown in water or. in 0.1 M NaCl. Seedling growth and cotyledon nucleic acid mobilization were delayed by NaCl salinity. The differences in cotyledonary RNase activity between seeds sown in water and in NaCl solutions suggest that salinity delays the activation and/or de novo synthesis of the enzyme. Cotyledon extracts were subjected to gel filtration through Sephadex G‐100, and RNase activity measured. Only one cotyledonary RNase appeared during germination, and salinity did not induce any change in molecular weight of the enzyme. Salinity inhibited 45% of the specific activity of the RNase on the 5th day of the experimental period. The same salt concentration (0.1 M NaCl) added in vitro inhibited only 8 % of the specific activity of the enzyme. This difference may indicate that NaCl in vivo affects mainly the de novo synthesis of the RNase.