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Analysis of [ 14 C] indole‐3‐acetic acid metabolites from the primary roots of Zea mays seedlings using reverse‐phase high‐performance liquid chromatography
Author(s) -
hebel Heather M.,
Crozier Alan,
Hillman John R.
Publication year - 1983
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1983.tb00742.x
Subject(s) - indole 3 acetic acid , decarboxylation , zea mays , acetic acid , incubation , high performance liquid chromatography , chromatography , chemistry , indole test , metabolism , auxin , biochemistry , biology , agronomy , catalysis , gene
Methanolic extracts of Zea mays L. cv. Fronica root segments which had been incubated in [ 14 C] indole‐3‐acetie acid were analysed by reverse‐phase high‐performance liquid chromatography. Metabolism of indole‐3‐acetic acid was found to be rapid and extensive with at least 11 products apparent after a 2 h incubation. A comparison of metabolites of [1‐ 14 C]– and [2‐ 14 C] IAA, calculations of 14 CO 2 evolution, and data on the polarity of products indicated that decarboxylation had not occurred. An average of 34% of the radioactivity remained associated with the indole‐3‐acetic acid peak.