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Influence of light on phosphoenol pyravate carboxylase in sorghum leaves
Author(s) -
Vidal Jean,
Godbillon Guy,
Gadal Pierre
Publication year - 1983
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1983.tb00741.x
Subject(s) - sorghum , phosphoenolpyruvate carboxylase , cycloheximide , enzyme , biochemistry , greening , pyruvate carboxylase , gene isoform , enzyme assay , chemistry , biology , botany , protein biosynthesis , agronomy , gene , ecology
Upon greening of sorghum leaves ( Sorghum vulgare Pers. cv. INRA 450) under white light illumination, phosphoenolpyruvate carboxylase activity increases. 17 times; at the same time, a new isoform of the enzyme appears. The aim of the present work has been to identify the process responsible for the appearance of this isoform. Greening. of the leaves in the presence of D 2 O did not lead to a significant increase in the buoyant density of the enzyme. On the other hand, cycloheximide was a powerful inhibitor of the rise in PEP carboxylase activity. In order to clarify these conflicting data a procedure based on the immunoprecipitation of the enzyme and its quantification by gel electrophoresis was developed in order to estimate the amount of enzyme in leaf tissue. The results clearly demonstrate that light triggers an increased synthesis of the enzyme protein during greening of sorghum leaves.