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Influence of light on phosphoenol pyruvate carb xoylase in sorghum leaves
Author(s) -
Vidal Jean,
Gadal Pierre
Publication year - 1983
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1983.tb00740.x
Subject(s) - sorghum , chemistry , etiolation , substrate (aquarium) , pyruvate carboxylase , enzyme , cellulose , ionic strength , biochemistry , food science , chromatography , botany , biology , agronomy , organic chemistry , ecology , aqueous solution
Chromatography on DEAE‐cellulose of an extract from etiolated leaves of sorghum ( Sorghum vulgare Pers. cv. INRA 450), a C 4 plant, gave only one form of phosphoenol pyruvate carboxylase with functional and regulatory properties of a C 3 type plant enzyme. Greening of the leaves resulted in a significant increase in activity. This increase was due to the appearance of a new form of the enzyme, which eluted at lower ionic strength and exhibited new properties. This form was glucose‐6‐P activated and showed a sigmoidal curve response to the concentration of the substrate phosphoerralpyruvate. These kinetic properties are typical of a C 4 plant enzyme.