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Uptake of radioactivity by Lemna gibba from 1– 14 C‐indole‐3‐acetic acid applied to the medium
Author(s) -
Bhatnagar Punam,
Tillberg Elisabeth
Publication year - 1982
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1982.tb06323.x
Subject(s) - lemna gibba , indole 3 acetic acid , lemna , chemistry , acetic acid , hydrolysis , botany , indole test , biochemistry , auxin , biology , aquatic plant , ecology , macrophyte , gene
Lemna gibba plants were incubated aseptically on medium containing labelled 10 ‐7 M indole‐3‐acetic acid (IAA‐1‐ 14 C). Most of the radioactivity disappeared from the culture medium during a 24 h light period. A high percentage of the loss was due to photolysis and only a low percentage of the radioactivity was recovered in the plants. Uptake of 14 C by the plants was strongly stimulated by light. The radioactivity taken up by the plants was the sum of photosynthetically taken up 14 CO 2 and 14 C taken up in IAA. Analyses with the indolo‐α‐pyrone fluorescence method revealed that the free IAA content was almost the same in plants grown in control and in IAA media for 5 h, whereas the amount of IAA which could be liberated by alkaline hydrolysis was doubled by the presence of IAA in the medium.