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Mitochondrial activity during shoot formation and growth in tobacco callus
Author(s) -
Brown Daniel C. W.,
Thorpe Trevor A.
Publication year - 1982
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1982.tb06314.x
Subject(s) - shoot , callus , subculture (biology) , nicotiana tabacum , primordium , biology , respiration , botany , succinate dehydrogenase , tissue culture , biochemistry , mitochondrion , in vitro , gene
Mitochondria isolated from tobacco ( Nicotiana tabacum L. cv. Wisconsin 38) callus growing on either shoot‐forming or non‐shoot forming medium show an increase in state 3 and state 4 respiration and a drop in respiratory control and ADP/O ratios after subculture. the protein content of the mitochondria fraction and the activity of succinate dehydrogenase, malate dehydrogenase, cytochrome c oxidase and catalase also increase after subculture but there is no apparent difference between shoot‐forming and non‐shoot‐forming tissue. For mitochondria assayed at their native osmolarities, a trend of higher respiration rates and respiratory control as well as lower levels of cyanide‐resistant respiration was observed for shoot‐forming tissue. Generally, differences were greatest after day 9 in culture, the time during which primordia formation occurred in the shoot‐forming callus. These patterns are in concert with the view that the shoot‐forming process has a high energy requirement which must be realized during the time of primordia formation.